| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
06/03/2017 |
|
Data da última atualização: |
29/03/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. |
|
Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
|
Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
|
Palavras-Chave: |
Boll weevil. |
|
Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
|
Thesaurus Nal: |
Cotton. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
|
|
Marc: |
LEADER 02841nam a2200241 a 4500
001 2066203
005 2023-03-29
008 2016 bl uuuu u01u1 u #d
100 1 $aSANTOS, R. C. dos
245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016
520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil.
650 $aCotton
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aBicudo
653 $aBoll weevil
700 1 $aSILVA, C. R. C. da
700 1 $aSOARES, T. da C
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aMONNERAT, R.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 24 | |
| 3. |  | MONNERAT, R.; MARTINS, E.; SILVA, C. R. C. da; LIMA, L. M.; ALBUQUERQUE, F. A. de; SANTOS, R. C. dos. Taxa de mortalidade de Spodoptera Frugiperda alimentadas com folhas de plantas transformadas (T0) de algodão contendo o gene CRY/1A. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Algodão. |
|   |
| 5. | | PEREIRA, J. W. de L.; CAVALCANTE, F. A.; SILVA, C. R. C. da; ilva1, DUARTE, E. A. A.; MELO FILHO, P. de A.; SANTOS, R. C. dos. Análise genética em acessos de amendoim ramador, do tipo Runner. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. 3 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Algodão. |
| |
| 6. | | CAVALCANTI, J. J. V.; SANTOS, R. C. dos; SILVA, C. R. C. da; CARVALHO, J. M. F. C.; LIMA, L. M. de; SOARES, T. da C. Breeding and crop improvement in cotton: expression of the serk gene in nonrecalcitrant cotton genotypes. IN: WORLD COTTON RESEARCH CONFERENCE, 6., 2016. Goiânia, Brazil. Proceedings... England, UK: Innovation in Textiles, 2016. p. 117| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
|  |
| 7. | | SANTOS, R. C. dos; PONTES, R. G. M. S. de; MARTINS, E. S.; LIMA, L. M. de; SILVA, C. R. C. da. Immunodetection and feeding bioassays as tools to identifying GM cotton resistant to insect. IN: WORLD COTTON RESEARCH CONFERENCE, 6., 2016. Goiânia, Brazil. Proceedings... England, UK: Innovation in Textiles, 2016. p. 132.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 8. | | SANTOS, R. C. dos; SILVA, C. R. C. da; LIMA, L. M. de; MELO FILHO, P. de A.; MARTINS, E. S.; RAMOS, A. R.; GOMES, R. M. S. Expressão do gene CRY1/A em plantas t0 de algodão oriundas da cv. BRS Antares por meio da técnica de microinjeção no ovário. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Algodão. |
| |
| 9. | | SOARES, M. M.; VASCONCELOS, E. D.; BRAZ, L. C. C.; RAMOS, J. P.; SILVA, C. R. C. da; SOFIATTI, V.; SANTOS, R. C. dos. Estimativa de mortalidade de Spodoptera frugiperda alimentadas com milho Bt (2B655PW) em ambiente semiárido. In: SIMPÓSIO DE ENGENHARIA DE BIOTECNOLOGIA E BIOPROCESSOS DO SEMIÁRIDO, 2., 2017, Sumé, PB. O papel da biotecnologia no uso da biodiversidade e no desenvolvimento sustentável: anais. Sumé, PB: UFCG, 2017. II SENGEBBIO.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
|  |
| 10. | | SILVA, M. de F. C. da; SOARES, T. da c.; FREIRE, R. M. M.; LIMA, L. M. de; SILVA, C. R. C. da; SANTOS, R. C. dos. Envolvimento do gene ARP (auxin repressed protein) na dormência de sementes de amendoim. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 66.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 11. | | SILVA, C. R. C. DA; RODRIGUES, J. D.; BARROS, R. V. A. M. DE; MELO FILHO, P. DE A.; LIMA, L. M. de; SANTOS, R. C. dos. Extração e análise eletroforética em gel de poliacrilamida (sds-page) de proteínas totais de folhas de algodão. In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO, 11., 2011, Recife. Anais... Recife: UFRPE, 2011. 2 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 12. | | SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. Relative expression of CRy10 in GM cotton lines resistant to boll weevil. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 13. | | RAMOS, J. P. C.; CAVALCANTI, J. J. V.; FREIRE, R. M. M.; SILVA, C. R. C. da; SILVA, M. de F. C. da; SANTOS, R. C. dos. Selection indexes and economic weights applied to runner-peanut breeding. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 26, n. 5, p. 327-334, 2022.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Algodão. |
| |
| 14. | | MONNERAT, R.; SANTOS, R. C. dos; LIMA, L. M. de; PINHEIRO, M. P. N.; SILVA, C. R. C. da; SOARES, C. M. Uso da transgenia para controle do bicudo-do-algodoeiro. In: O bicudo-do-algodoeiro (Anthonomus grandis BOH., 1843) nos cerrados brasileiros: biologia e medidas de controle. Cuiabá, MT: Instituto Mato-Grossense do Algodão, 2015. 254p. (Boletim de P&D, 2). p. 178-206| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 15. | | SOARES, T. da C.; LIMA, L. M. de; SILVA, C. R. C. da; SANTOS, I. L. V. de L.; CARVALHO, J. M. F. C.; MELO FILHO, P. de A.; SANTOS, R. C. dos. Análise in sílico da homologia do gene Serk em Gossypium hirsutum L. e espécies correlatas. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| 16. | | SILVA, R A. da; RAMOS, J. P. C.; LUZ, L. N. da; CAVALCANTI, J. J. V.; LIMA, L. M. de; FREIRE, R. M. M.; SILVA, C. R. C. da; SANTOS, R. C. dos. Assessment of genetic divergence in runner peanut genotypes grown in the Brazilian Northeast environments. African Journal of Agricultural Research, v. 11, n. 16, p. 1456-1462, Apr. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Algodão. |
| |
| 17. | | LIMA, L. M. de; SOUZA, C. C. F. de; PINHEIRO, M. P. N.; SILVA, C. R. C. da; BATISTA, V. G. L.; SANTOS, M. M. S.; DUARTE NETO, J. M. W.; SANTOS, R. C. dos. Análise molecular de plantas de algodão BRS Araçá submetidas a transformação via ovary-drip. In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Algodão. |
| |
| 19. | | SANTOS, R. C. dos; DUARTE, M. de M. F.; SILVA, M. DE F. C. DA; BRAZ, L. C. C.; SILVA, C. R. C. DA; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. Initial assessment of GM cotton resistant to cotton boll weevil based on feeding bioassays and Elisa. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 20. | | SANTOS, R. C. dos; DUARTE, M. de M. F.; SILVA, M. DE F. C. DA; BRAZ, L. C. C.; SILVA, C. R. C. DA; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. Initial assessment of GM cotton resistant to cotton boll weevil based on feeding bioassays and Elisa. In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
| |
| Registros recuperados : 24 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
